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After washed twice with PBS in the plates, cells were removed from the plate by scraping twice with each 0.3 ml BHM solution consisting of 0.32 M sucrose, 1 mM EDTA, 10 mM Tris-Cl (pH 7.0) and the complete protease inhibitor cocktail (Roche Applied Science, Upper Bavaria, Germany)

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The fat-binding capacity was calculated using the following the formula

Expecting overnight results: Glucagon-pathway effects discussed in research often need several weeks to show clearly

Appl Microbiol Biotechnol 103(3):10811094 Tan J, McKenzie C, Potamitis M, Thorburn AN, Mackay CR, Macia L (2014) The role of short-chain fatty acids in health and disease