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At certain intervals from Day 4 to the cell recovery date, add IFN- to added new medium in proportion as cells grow (if necessary, expand the culture scale with T-Flasks and gas-permeable culture bags.) Dilute the cell suspension above by adding fresh medium to a cell concentration of 12 10 cells/mL

Brcier A, Li VW, Smith CS, Halievski K, Ghasemlou N

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