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The total and separated extracts were subjected to RNA extraction using Trizol reagent (15596026, Thermo Fisher Scientific, USA) 59

Rappaport AR, Hong SJ, Scallan CD, Gitlin L, Akoopie A, Boucher GR, et al

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Here, these fatty acids oxidize, releasing energy

Preclinical evidence suggests that increasing protein intake may offset circulating amino-acids reduction, however this may also blunt the increase in whole-body energy expenditure [63]

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