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Immunofluorescence To assess cell area and morphology, and for quantification of p-FAK, Ki67, MITF, YAP, and p-ERK signal intensities, MCs were seeded on the different substrate combinations at a density of 2500 cells/cm, to avoid overcrowding and allow single cell measurements

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It is commonly utilised in laboratory environments to investigate G-protein coupled receptor (GPCR)-mediated steroidogenic signalling and endocrine system dynamics