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Lyophilised (Pre-Reconstitution) Storage Store at 20C in a frost-free freezer, away from light and moisture Avoid repeated freeze-thaw cycles this degrades peptide structure Keep sealed in the original vial until ready for use Lyophilised peptides are stable at room temperature for short periods during transit resume cold storage immediately on receipt Reconstitution Use bacteriostatic water or sterile saline inject solvent slowly down the side of the vial, do not shake Allow powder to dissolve gently do not vortex Use sterile technique throughout sterile needles, sterile vials, gloves Post-Reconstitution Storage Store reconstituted solution at 28C (standard refrigerator) Use within 28 days of reconstitution Do not refreeze reconstituted peptide solution Protect from light amber vials or foil wrapping recommended 9

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Photosynth Res 86:435457 Mhamdi A, Hager J, Chaouch S, Queval G, Han Y, Taconnat L, Saindrenan P, Gouia H, Issakidis-Bourguet E, Renou JP, Noctor G (2010) Arabidopsis GLUTATHIONE REDUCTASE1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways

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1e and Supplementary Fig