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Intracellular accumulation of unconjugated bilirubin inhibits phytohemagglutin induced proliferation and interleukin-2 production of human lymphocytes
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The cells were incubated with the WST-1 reagent for 1 h at 37 C in the dark and the absorbance of the WST-1 formazan product was measured at 450 nm wavelength using a microplate reader (Bio-Rad)
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