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Shorter time period: 45 minutes or less (NAD+ may take up to 2 hours)

Real-time RT-PCR Request a detailed protocol Quantitative real-time PCR was performed using a Rotor-Gene 3000 system (Corbett Research) with a QuantiTect SYBR Green RT-PCR kit (Bio-rad) as described (Sun et al., 2011)

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