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With the increasing F1 peptide concentrations, from 0.8 to 3.2 M, the V max value decreased from 0.833 mol/mg/min to 0.645 mol/mg/min, K i value increased from 4.0 M to 5.82 M, and [I]/K i value increased from 0.2 to 0.55
Free lysine does not serve as the precursor for this reaction, rather the modified lysine ( 6- N -trimethyllysine , TML), found in certain proteins, is the precursor
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