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Ann Neurol 2018;84:854-72

Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)

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Never assign these codes based on assumption the route must be explicitly documented

The recombinant SOD (e.g., obtained from mice milk) has a short plasma half-life when injected into animals (Halliwell and Gutteridge, 2015)