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The protein samples were subjected to 10% or 12% SDSPAGE, transferred to PVDF membranes (Millipore) and incubated with an antibody against tubulin (1:1000), p16 Ink4a (1:1000), p21 (1:1000), Tenascin (1:500), Thbs2 (1:500), Laminin1 (1:1000), Fibronectin (1:1000), Hmmr (1:500), SMA (1:1000), Col1a1(1:1000) and FOXO4(1:500)

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