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The following antibodies were used: cytokeratin 7 (CK7/KRT7) (Abcam, ab181598, 1:1000), CYP3A4 (Invitrogen Antibodies, MA5-17064, 1:1000), desmin (R&D Systems, AF3844, 1:20), and albumin (Fortis Life Sciences, A80-129 A, 1:1000)

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MTHFR variants and methylation dysfunction have been implicated in: Depression and anxiety Bipolar disorder Schizophrenia Chronic fatigue and fibromyalgia Recurrent pregnancy loss and infertility Cardiovascular and cerebrovascular disease Neurodegenerative disorders such as Alzheimers disease Supporting methylation through targeted nutrition and appropriate forms of folate can improve neurotransmitter synthesis, reduce oxidative stress, and enhance overall neurological stability

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