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However, for most people, a single, well-timed dose is sufficient and much easier to maintain as a long-term habit

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After filtration, centrifugation, precipitation, and resuspension, FLS were cultured in complete culture media (DMEM with 10% fetal bovine serum, 1% antibiotics) and maintained at 37 C in a humidified incubator of 5% CO 2 and 95% air