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(8) The increased IGF-1 may act on Leydig cells via the IGF-1 receptor, which is thought to be present on these cells, and has been posited to support their responsiveness to gonadotropins

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Similarly, studies in aged rats showed that sevoflurane and isoflurane contributed to PND by promoting the release of proinflammatory cytokines and activating microglia in the brain (Peng et al., 2012

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More aggressive approaches might use three cycles annually, though research supporting increased frequency remains sparse