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Cell culture The human ovarian granulosa cell line KGN (Procell, Wuhan, China) was cultured in Dulbecco's modified Eagle's medium (DMEM)-F12 medium (GIBCO-BRL, Invitrogen) containing 10% fetal bovine serum (Invitrogen) and 1% penicillinstreptomycin (Invitrogen) at 37C under 5% CO

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3 Park JS, Kam TI, Lee S, et al