Supplementary Video 2 (download AVI Live imaging of representative macrophages infected with an inducible mKate reporter S
Lemieux GA, Cunningham KA, Lin L, Mayer F, Werb Z, Ashrafi K
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Specifically, research has found GHK-Cu downregulates genes involved in: Metastasis and invasion (MMP-2, MMP-9) Tumor angiogenesis (distinct from healthy tissue angiogenesis it supports) Cancer stem cell maintenance The proposed mechanism is that GHK-Cu may restore normal cellular differentiation programs that cancer cells have dysregulated
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The column was washed with 25 mM Tris (pH 7.5), 200 mM NaCl and 10 mM imidazole, and eluted with 25 mM Tris (pH 7.5), 200 mM NaCl and 500 mM imidazole