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A key challenge lies in its interaction with drug-metabolizing enzymes, particularly cytochrome P450 enzymes, particularly CYP3A4, which is involved in the metabolism of many drugs [12, 116, 117]

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Subsequently, immunostaining was visualized with diaminobenzidine and slices were counterstained with hematoxylin

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Approximately 95% of this bile acid pool is actively reabsorbed in the distal ileum, while around 5% is excreted in the feces