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doi:10.1016/j.rvsc.2023.05.007

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To better understand the role of W33 in small-molecule agonist binding to GLP-1R, we generated the cryo-EM structure of a PF-06882961 analog (PF-06883365) bound to human GLP-1R (Fig

After DNA extraction and amplification, the PCR product was loaded on 1% agarose gels (UltraPure Agarose, Invitrogen, Thermo Fisher Scientific), then separated for 30 minutes at 150 V (Owl Easycast B2 Separating System), and the bands were visualized under a blue light transilluminator (UVP GelDoc-It2 Imager)

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Neither medication alone delivered both benefits optimally