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Were talking 23 months, sometimes even more

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Data were acquired using an MS3-based TMT method using the following scan parameters: scan sequence began with an MS1 master scan (Orbitrap analysis, resolution 120,000, 4001700 m/z, RF lens 60%, automatic gain control [AGC] target 2E5, maximum injection time 50 ms, centroid mode) with dynamic exclusion enabled (repeat count 1, duration 15 s)

Among them, 3-HSD was capable of converting dehydroepiandrosterone to androstenediol.CYP19 catalyzes the conversion of androstenedione and testosterone into estrone and estradiol, respectively, and is the rate-limiting enzyme in estrogen biosynthesis