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After 2 d of treatment, the zebrafish were transferred to a 96-well plate, allowed to acclimate for 5 min, and then permitted to swim freely under light conditions for 15 min ( n = 10 biological replicates, with each replicate consisting of one zebrafish larva, from three independent experiments)

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The expression of CHGA remained stable throughout the trajectory, suggesting that endocrine cell identity was preserved (Supplementary information, Fig

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