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11 The preproglucagon gene is located on chromosome 2 (2q36-q37) spanning an estimated 9.4kb comprising of six exons and five introns, and exons 2 to 5 encode the proteins with known biological roles: glucagon is encoded in exon 3

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Interscapular BAT (iBAT) and epididymal WAT (eWAT) were collected, weighted, and subjected to hematoxylin and eosin (H&E) staining or snap-frozen in liquid nitrogen and stored at 80C until analysis

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