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The dough was rolled to a 3 cm thickness and then transferred to a baking tray (30 cm 30 cm)

The coverslips were treated with 0.5% TritonX-100 at room temperature for 20 min and blocked with 1% BSA at 37 C for 1 h

We remove all impurities and the non dissociated colloidal matter, gas and organic matter in the water to a very low level

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