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J Manag Care Pharm 12 (S212), quiz S1416 (2006)

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Effects of selenium and iodine deficiency on bone, cartilage growth plate and chondrocyte differentiation in two generations of rats

The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (EM1901-56) at 1/2,000 dilution for 1 hour at room temperature