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PCR products of between 400 and 450 bp were selected for PCR product purification using GeneJET (Thermo Scientific, Waltham, MA, United States) following the manufacturers instructions

We believe that this method can be used as a common tool for finding enzyme fragments that interact with a substrate, and subsequently for finding enzyme inhibitors

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Studies of animal models have shown that such heterozygotes have a higher incidence of cardiomyopathy with aging and may be at risk for cardiac abnormalities when additional cardiac burdens are imposed

These data differ from the indications of other authors who observed an increase in serum cortisol concentration during maximum intensity exercise, performed on an ergometer and a treadmill, regardless of nutritional status 36