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meanwhile, the produced Fe 3+ was reduced by GSH into Fe 2+ , accompanying the generation of GSSG (Figures 3A, B)
The cells were incubated overnight (approximately 16 h), and then 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)
Acknowledgements We thank the staff of the Animal Magnetic Resonance Research Center of the First Affiliated Hospital of Jinan University for performing the animal MRI scans
2-3 ml of Plasmagel was added to 5 ml of heparinized whole blood by Song et al [8]
All of this is very interesting but no matter what the outcome, it is still an experiment of N=1 that cannot be extrapolated to others