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Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV

Glutathione and Detoxification Because of glutathiones central role in detoxification, about 25% of all glutathione resides in the liver

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This can lead to impairment of the glymphatic system, resulting in reduced clearance and increased aggregation of A and tau, exacerbating AD pathology 39 , 40

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Chronic conditions may require 2-4 weeks before meaningful changes appear