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Pirfenidone and risk of lung cancer development in IPF: a nationwide population-based study

What is measured in these rats and mice (and occasionally in small groups of human volunteers) is often not a hard endpoint like muscle strength or weight loss, but surrogate biomarkers: molecules in the blood that have an indirect link to a hard endpoint

We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

Although one study reported a higher level of DAT in the striatum of SHR rats than WKY rats when they were 2-week old [62], overall no significant difference in the levels of D2R or DAT was reported between SHR and WKY rats

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[DOI] [PMC free article] [PubMed] [Google Scholar] 449