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It has been reported that silencing of PTEN cooperates with activated AKT to promote metastasis of melanoma (102, 103, 106, 107), but MM does not show a weaker performance in metastasis than CM in the clinic possibly because there are other gene alterations in the PI3K pathway promoting invasiveness of tumor (108)

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Reduced histidine was correlated with Expanded Disability Status Scale values

Samples were fixed in 4% paraformaldehyde, followed by sucrose dehydration, embedding, and transverse cryosectioning