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Generation of the magnetic bead-capture antibody complex and HRP-conjugated antibody To immobilise biotinylated antibody on streptavidin-coated magnetic beads, 2 L (2 g) of biotin-conjugated mouse anti-influenza A virus NP clone A3 and 10 L (10 8 beads) of magnetic beads per reaction were mixed in 1.5 mL Eppendorf tubes and incubated at room temperature for 30 min