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Starting Point: Gathering Baseline Data First things first - you need to know where you're starting to measure true progress

T.LeachK.StanleyD.LuongS.et al (2015)

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Comparison of plasma levels and analgesic effect between oral transmucosal and subcutaneous administration of buprenorphine in rabbits

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The content was 93% higher in the 35S::MrMYB1.1 + 35S:: MrbHLH1 + 35S::MrGST1 injected areas than 35S::Mr MYB1.1 + 35S::MrbHLH1 injected areas (Figure 5B), implicating that MrGST1 is a rate-limiting step for anthocyanin accumulation