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3rd Edn.Chichester, UK: Wiley and Sons

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The assay mix contained 30 mM glycerol 3-phosphate (substrate 1), 30 ng mL 1 of sp PlsY, 20 M of palmitoyl phosphate (substrate 2), and 5 M MDCC-PBP in the buffer containing 0.03%(w/v) LMNG, 0.15 M NaCl, 50 mM Tris-HCl pH 8.0