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Similar content being viewed by others Introduction Nicotinamide adenine dinucleotide (NAD + ) is an essential redox coenzyme that exists between the oxidized form NAD + and its reduced form (NADH) 1

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LDH activity was determined spectrophotometrically at 340 nm by following the rate of NAD + reduction in the presence of l-lactate

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Goldstein and colleagues described it as a component of the thymosin fraction from calf thymus, and later work demonstrated its ubiquitous expression in nucleated mammalian cells and high concentration in platelets [1]

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