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Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

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Once reconstituted in sterile water or buffer, store at 4 C for short-term use (up to one week) or aliquot and freeze at -80 C to minimize copper dissociation and peptide degradation

So, if you were to take a T3-containing thyroid medication a few hours before your thyroid lab tests, your lab results might show that you have too much T3, even when you may be accurately dosed

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