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After staining, the samples were imaged using an inverted microscope (Nikon, Japan), and the areas of positive staining were quantified using ImageJ software (National Institutes of Health, USA, to determine the relative areas of calcium nodules, lipid droplets, and cartilage matrix

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Genetic isolated GHD (IGHD) can be an alternative way to assess the biological impacts of GH ( GHR gene and congenital IGF1 appear to be protected against the development of neoplasms