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Cytokine production by adherent and non-adherent mononuclear cells in chronic fatigue syndrome
We also evaluated melatonins impact on mitochondrial membrane potential using TMRM
Western Blot Analysis After drug treatment, HT22 cells or hippocampal tissues were harvested and lysed in RIPA buffer supplemented with protease and phosphatase inhibitor
Immunohistochemical staining was performed for TNF-, IL-6, and IL-10 to evaluate the inflammatory response in the tissue, VEGF and CD31 to evaluate angiogenesis, and Ki67 to assess cell proliferation