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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)

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The study identifies butyrate supplementation as a readily translatable strategy to counteract gut-derived, BMP-induced neurodegeneration

Complexes were induced by the addition of high concentrations of agonist (10M GLP-1 or 50M for small molecules) prior to solubilisation and purification