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Luciferase assay To directly analyze the transcriptional regulation of SAA enzyme expression by HNF4, firefly luciferase reporters driven by the human MAT1A (NG_008083, 1684-1890), BHMT (NG_029156, 2128-2282), and CBS (NG_008938, 12674-12911) promoters containing either wild type (WT) or mutant HNF4 binding site were cloned into pGL3 basic vector

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In trials in which adult patients were randomized prior to dose-escalation, more patients treated with WEGOVY, compared with placebo, had maximum changes from baseline at any visit of 10 to 19 bpm (41% versus 34%, respectively) and 20 bpm or more (26% versus 16%, respectively)