Przechowywanie w temperaturach ujemnych po uwodnieniu wywouje zjawisko krystalizacji fazy wodnej, co prowadzi do mechanicznej denaturacji i nieodwracalnego uszkodzenia struktury acucha aminokwasowego
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125 GCGR knockout mice produced in one study had islet alpha-cell hyperplasia and these mice were additionally shown to have large increases of glucagon and GLP-1 levels in circulation, but interestingly they had lower plasma glucose levels throughout the day, similar insulin levels and improved glucose tolerance compared to GCGR+ control animals