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For longer analyses, maintaining a constant temperature is essential to ensure reproducibility and stability of results ( Lysimachia nummularia , and Acacia species ( 3.2.4 Other methods for polyphenol quantification In addition to widely used techniques, several other methods are employed to identify plant-derived bioactive compounds, including capillary electrophoresis, paper chromatography, supercritical fluid chromatography, spectrophotometric assays, HPLC, and gas chromatography ( However, paper chromatography is used less frequently than HPLC and gas chromatography due to its limited sensitivity and specificity ( Senna spectabilis ( Supercritical fluid chromatography is an advanced method increasingly used for the analysis of complex plant materials ( 4 Bioavailability of polyphenols Bioavailability refers to the proportion of polyphenol-derived nutrients that are consumed, absorbed, and metabolized ( Polyphenols are also subject to various denaturing conditions ( Cannabis sativa L
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