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doi: 10.3390/ijms23147848 54 KanderMCCuiYLiuZ

Plasmid-based gene therapy with hepatocyte growth factor stimulates peripheral nerve regeneration after traumatic injury

Registered nurses screen for contraindications and monitor throughout treatment

The following recommendations apply specifically to injectable (subcutaneous) GHK-Cu

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ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C