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In those cases, your bodys natural antioxidant supply may not be able to keep up

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Plasmids were transfected into SH-SY5Y cells at 6080% of the culture plate by Lipo8000 (Beyotime, C0533), and protein were extracted with RIPA lysate for subsequent experiments 48 h after transfection

It is interesting to note that D- as well as L-methionine could restore growth, implying that depletion of sulfur was the primary defect and not one related to protein synthesis