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Quality control (QC) samples were prepared by spiking fresh rat plasma with an appropriate dilution of dihexa for the final concentration of dihexa specified, keeping a 10: 1 ratio of plasma to dihexa solution (final DMSO concentration 5%)

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doi: 10.3389/fendo.2025.1630164 Received 17 May 2025 Accepted 09 July 2025 Published 28 July 2025 Volume 16 - 2025 Edited by Nazli Akin, Vrije University Brussels, Brussels, Belgium Reviewed by Yuan Gao, Gansu Agricultural University, Lanzhou, China Hong-Thuy Bui, International University, Vietnam Updates Copyright 2025 Zhong, Lu, Zhou, Ma, Li, Hu, Wan and Lu