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1.2 10 5 SHSY-5Y cells were subjected to similar treatment as above except 1 mM MPP + (for 24 h) was used to induce ROS

Integrating in vitro, , network pharmacology, and molecular docking approaches to uncover the antidiabetic potential of Tylophora hirsuta

These melanin-inhibiting and detoxifying antioxidants work synergistically and can be administered either in combination or in sequence

PMID 26288227

In an oral form standardized to contain 70 to 80 percent silymarin, milk thistle appears to be safe for up to 41 months of use

Following, the cells were washed two times by centrifugation in PBS (450g for 5 min)