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In most cases, freshly boiled extracts were processed further as described below or, in some cases, lysates were stored frozen at 30 C

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To obtain cell surface proteome lists for T cell, CAR-T cell and TCR-T cell monocultures (grown in the absence of tumor cells), we applied a log 2 -transformed fold change cutoff with a 5% false discovery rate (FDR), defined by the retention of cytosolic and nuclear proteins, which should not be labeled by LaccID (Supplementary Fig