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The pattern of injections described and illustrated below are of the same PREEMPT protocol

These are general guidelines

6b) While this would impair killing of VSMC and VEC, which could help prevent ASCVD progression, it would similarly prevent CD8 + T cells from suppressing inflammatory CD4 + T cells, APCs that promote immune activation, and plasma cells that produce inflammatory IgG 2,4

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After 96 h, the cells were harvested using pancreatic enzymes, washed twice with PBS, and lysed with 1% TritonX-100 (300 L for 0 M CGA and 150 L for 600 M CGA) on ice for 10 min

After air-drying, the incorporation of [- 32 P] was quantified using a LS6500 multi-purpose liquid scintillation counter (Beckman Coulter, Brea, CA) via Cerenkov counting