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Rapid weight loss and reduced nutrient intake may lead to some bone density reduction

RNA quality and quantity were determined using the RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA)

Some patients continue on low-dose maintenance therapy

Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP

Most people notice a smaller appetite within the first week and gradual weight loss over the next 1-3 months

In current clinical practice, emphasizing the combination of adequate protein intake and regular resistance exercise during treatment is a key strategy for monitoring and reducing lean body mass loss and maximizing treatment benefits