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Reconstitute a GLOW-70 vial by adding 2.5 mL of BAC water That delivers a concentration of 28 mg/mL The resulting dose per injection: 2.8 mg 28 mg/mL = 0.1 mL or 10-units on a U-100 insulin syringe Each 70 mg vial of GLOW supplies 25 daily doses and remains within the 28-day refrigerated-use window

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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100