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Immunohistochemistry and immunofluorescence analyses further corroborated these observations, showing trends consistent with the protein expression results (Fig

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Upon stimulation by bronchoconstrictor agonists, there is an increase in intracellular calcium concentration ([Ca 2+ ]), triggering Ca 2+ calmodulin binding, MLCK activation, and actinmyosin interactions that ultimately result in contraction (Zhu, 2011)

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