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UVvis spectra were acquired using a Multiskan SkyHigh microplate spectrophotometer (ThermoFisher Scientific) and 96-well plates (Corning) or quartz cuvettes using SkanIt software

Zhang Y, Rahematpura S, Ragnini KH, Moreno A, Stecyk KS, Kahng MW, Milliken BT, Hayes MR, Doyle RP, Schmidt HD

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The function of metformin in upregulating GLP-1R level and PKA phosphorylation in endothelial cells may contribute to the synergistic protective effect of this combination therapy, which provides new insights on the interaction between metformin and GLP-1 agents (Fig