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Subsequently, we performed mRNA stability assays by treating cells with actinomycin D and found that GGCT mRNA degradation rate was significantly accelerated under 0 mM Gln compared with 4 mM Gln, indicating that Gln regulates GGCT in a post-transcriptional manner (Fig
The data validation process included: Standardization of units (currency conversions, volume units, inflation adjustments) Cross-verification of data points across multiple secondary sources Normalization of inconsistent datasets Identification and resolution of data gaps Outlier detection and removal through algorithmic and manual checks Plausibility and coherence checks across segments and geographies This ensured that the dataset used for modelling was clean, robust, and reliable
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doi: 10.1038/s41421-022-00389-z
