The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA
Then, the cells were washed with PBS and lysed with PTTS buffer (0.5% Triton-100, 0.1% Tween-20, and 0.05% SDS) containing a protease inhibitor (Cat
We hypothesized that exposures to environmentally relevant concentrations of BPA during critical periods in visual system development would directly affect the retina and be correlated with altered visual behavior and physiology
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10.3109/10715769609149065 107 RaniB.DhawanK.JainV.ChhabraM